王海龙

作者: | 来源: | 发布日期:2019-02-27 | 阅读次数:

 

王海龙(Hailong Wang)博士、教授

 

电话: 010-68901494

 

Email: Hailwang@cnu.edu.cn

whlpost@hotmail.com

 

研究生招生方向:生物化学与分子生物学

研究生招生方向:肿瘤发生的分子机制

研究方向

    遗传信息的精准复制和传递对于维持正常的生命活动是必须的。细胞基因组DNA不断受到内源性(如活性氧自由基和细胞代谢中间产物)和外源性(如电离辐射、化学致癌物质、紫外线等)因素的影响而发生损伤。生物体进化出了一套复杂而精细的 DNA损伤响应机制,用于修复损伤的 DNA,维持基因组的稳定性。 DNA 损伤修复缺陷与异常生命活动如细胞突变、衰老、肿瘤发生都有密切的关系。本课题组用哺乳动物细胞作为模型系统,研究细胞响应及修复DNA双链断裂损伤的分子机制。并以此为切入点,探索肿瘤等疾病发生的分子机制,为临床诊断、治疗策略及药物研发提供理论指导。

    欢迎有志于生物医学基础研究的同学报考及调剂。

教育经历

1995-1999        北京师范大学 生命科学学院

2002-2007        北京师范大学 生命科学学院 

2008-2013   博士后   美国斯克利普斯研究所

The Scripps Research Institute, La Jolla, California

工作履历

2019-今      教  授   首都师范大学生命科学学院

2013-2018    副教授   首都师范大学生命科学学院

1999-2007    实验师   北京师范大学生命科学学院

 

主持科研项目

国家重大科学研究计划(973)子课题:146万元,2015-2019  

北京市自然科学基金面上项目: 20万,2018-2020  

国家自然科学基金面上项目: 80万,2014-2017

北京市属高等学校青年拔尖人才培育计划,30万,2015-2017 

首师大燕京学者培育计划: 20万 2015-2017

 

获  奖

国家教育教学成果奖二等奖:2005,第三完成

北京市教育教学成果奖一等奖:2004,第三完成

国家级精品课程:2004,第三完成

北京市精品课程:2004,第三完成

全国高校生命科学类微课教学比赛二等奖: 2016,独立完成

 

代表性论文:

 

1. Wang, H#., Li, S#., Zhang, H., Wang, Y., Hao, S., and Wu, X*. (2018) BLM prevents instability of structure-forming DNA sequences at common fragile sites. PLoS genetics 14, e1007816

2. Wang, H*., Qiu, Z., Liu, B., Wu, Y., Ren, J., Liu, Y., Zhao, Y., Wang, Y., Hao, S., Li, Z., Peng, B., and Xu, X*. (2018) PLK1 targets CtIP to promote microhomology-mediated end joining. Nucleic acids research 46, 10724-1073

3. Wang, H#., Li, S#., Oaks, J., Ren, J., Li, L. and Wu, X*. (2018) The concerted roles of FANCM and Rad52 in the protection of common fragile sites. Nature communications, 9, 2791.

4. Wang, H*., and Xu, X*. (2017). Microhomology-mediated end joining: new players join the team. Cell & bioscience 7, 6

5. Wang, H#., Li, Y#., Truong, L. N., Shi, L. Z., Hwang, P. Y., He, J., Do, J., Cho, M. J., Li, H., Negrete, A., Shiloach, J., Berns, M. W., Shen, B., Chen, L., and Wu, X*. (2014) CtIP maintains stability at common fragile sites and inverted repeats by end resection-independent endonuclease activity. Molecular cell   54, 1012-1021.

6. Hailong Wang, Linda Z. Shi, Catherine CL Wong, Xuemei Han, Patty Yi-Hwa Hwang, Lan N. Truong, Qingyuan Zhu, Zhengping Shao, David J. Chen, Michael W. Berns, John R. Yates III, Longchuan Chen and Xiaohua Wu* (2013). The interaction of CtIP and Nbs1 connects CDK and ATM to regulate HR-mediated double-strand break repair. Plos Genetics.

7. Wang, H., Shao, Z., Shi, L.Z., Hwang, P.Y., Truong, L.N., Berns, M.W., Chen, D.J., and Wu, X*. (2012). CtIP dimerization is critical for its recruitment to chromosomal DNA double-strand breaks. The Journal of biological chemistry

8. Wang, H., Du, Y., Xiang, B., Lin, W., Li, X., and Wei, Q*. (2008). A renewed model of CNA regulation involving its C-terminal regulatory domain and CaM. Biochemistry 47, 4461-4468.

9. Wang, H., Yao, S., Lin, W., Du, Y., Xiang, B., He, S., Huang, C., and Wei, Q*. (2007). Different roles of Loop 7 in inhibition of calcineurin. Biochemical and Biophysical Research Communications 362, 263-268.

10. Wang, H.L., Du, Y.W., Xiang, B.Q., Lin, W.L., and Wei, Q*. (2007). The regulatory domains of CNA have different effects on the inhibition of CN activity by FK506 and CsA. IUBMB Life 59, 388-393.

11. Peng, B., Wang, J., Hu, Y., Zhao, H., Hou, W., Zhao, H., Wang, H., Liao, J., and Xu, X*. (2015) Modulation of LSD1 phosphorylation by CK2/WIP1 regulates RNF168-dependent 53BP1 recruitment in response to DNA damage. Nucleic acids research 43, 5936-5947

12. Makharashvili, N., Tubbs, A. T., Yang, S. H., Wang, H., Barton, O., Zhou, Y., Deshpande, R. A., Lee, J. H., Lobrich, M., Sleckman, B. P., Wu, X., and Paull, T. T*. (2014) Catalytic and noncatalytic roles of the CtIP endonuclease in double-strand break end resection. Molecular cell 54, 1022-1033

13. Truong, L. N., Li, Y., Shi, L. Z., Hwang, P. Y., He, J., Wang, H., Razavian, N., Berns, M. W., and Wu, X*. (2013) Microhomology-mediated End Joining and Homologous Recombination share the initial end resection step to repair DNA double-strand breaks in mammalian cells. PNAS 110, 7720-7725

14. Ye, Q., Wang, H., Zheng, J., Wei, Q., and Jia, Z*. (2008). The complex structure of calmodulin bound to a calcineurin peptide. Proteins Structure Function and Bioinformatics 73, 19-27.

15. Ye, Q.L., Wang, H.L., Wong, A., Li, X., Wei, Q., and Jia, Z.C*. (2007). Crystallization characteristics of calmodulin in complex and fused with calcineurin peptide. Cryst Growth Des 7, 2198-2201.