萧伟

作者:生命科学院 | 来源: | 发布日期:2012-09-06 | 阅读次数:
    萧伟,博士生导师,国家“千人计划”教授。
 
    电话:+86-10-68903412
   
    传真:+86-10-68909617
  
    电子信箱:weixiao@mail.cnu.edu.cn
                            wei.xiao@usask.ca
                                 

教育背景:
1. 学士/1982 南京农业大学
2. 硕士/1984 University of Toronto
3. 博士/1988 University of Saskatchewan
4. 博士后/1990-1992 Harvard University

工作经历:
1.1992-1995 萨斯卡切温大学医学院微生物与免疫学系,助理教授
2.1995-2000 萨斯卡切温大学医学院微生物与免疫学系,副教授
3.2000至今 萨斯卡切温大学医学院微生物与免疫学系,教授
4.2003-2008 萨斯卡切温大学医学院微生物与免疫学系,系主任
5.2009至今,首都师范大学生命科学学院,国家“千人计划”教授,院长

获得荣誉及获奖情况:
1.萨斯喀彻温大学年度最佳研究奖(2008)
2.萨斯喀彻温大学医学院研究生会(CMGSS)年度最佳导师奖(2007)
3.人物传记被收录于《千禧年的加拿大》(CANADA at the Millennium)(2000)
4.人物传记被收录于《加拿大名人录》(Canadian Who’s Who)(自1996)

研究领域:
    泛素(UbiquitinUb是一种高度保守的小蛋白(76个氨基酸),普遍存在于从单细胞酵母到人类的所有真核细胞内,并且表达水平很高。通过一系列的酶促反应,泛素在泛素结合酶(E2或Ubc)和泛素连接酶(E3)的帮助下连接到目的蛋白。接着在一个被称为多聚泛素链的泛素多聚体形成后,结合有多聚泛素链的目的蛋白被26S蛋白酶体降解,而这个过程是许多细胞活动调节的重要手段,2004年的诺贝尔奖授予了“泛素化的发现和它在蛋白降解中的作用”就说明了它的重要性。深入研究揭示,上述泛素链是由下一个泛素表面的Lys48残基结合在上一个泛素的C末端甘氨酸残基而形成的,随后又发现泛素链也可经由表面Lys63残基形成。在所有生物中发现的泛素结合酶中,只有Ubc13能够专一通过Lys63连接泛素。Ubc13的独特性质是基于它和另一个被称作泛素结合酶异构体的蛋白的结合,而这种异构体也是Lys63多聚泛素化过程所必须的。更重要的是,被Lys63连接的泛素所结合的目的蛋白不是为了降解,而是为了改变其活性。此外,在其他泛素化类型中,单一泛素化也被认为是一种新颖的蛋白质活性调节机制,拓宽了人们对泛素化功能的认识。本实验室的主要兴趣在于发现上述非常规泛素化过程和探索相关途径的分子机制。因为一些研究的很透彻的非常规泛素化目的蛋白参与一些重要的细胞代谢过程,如DNA损伤耐受、细胞周期检验点、自然免疫和应激反应,而且这些过程在人类疾病中起关键作用,如癌症,抗病及衰老,所以这项研究将会对包括癌症在内的多种疾病诊断和治疗产生直接影响。
硕士招生:生物化学与分子生物学  泛素生物学
博士招生:生物化学与分子生物学  泛素生物学
 
 

发表论文
 

1.           Xue, C., Liang, K., Liu, Z., Wen, R. and Xiao, W. (2015) Similarities and differences between Arabidopsis PCNA1 and PCNA2 in complementing the yeast DNA damage tolerance defect. DNA Repair 28: 28-36. [Text]

2.           Li, S., Pan, X., Berry, J.O., Wang, Y., Naren, Ma, S., Tan, S., Xiao, W., Zhao, W., Sheng, X. and Yin, L. (2015) OsSEC24, a functional SEC24-like protein in rice, improves tolerance to iron deficiency and high pH by enhancing H+ secretion mediated by PM-H+-ATPase. Plant Sci. 233: 61-71. [Text]

3.           Rout, M.K., Hodge, C.D., Markin, C.J., Xu, X., Glover, J.N.M., Xiao, W. and Spyracopoulos, L. (2014) Stochastic gate dynamics regulate the catalytic activity of ubiquitination enzymes. J. Am. Chem. Soc. 136: 17446-17458. [Text]

4.           Ball, L.G., Hanna, M.D., Lambrecht, A.D., Mitchell, B., Ziola, B., Cobb, J.A. and Xiao, W. (2014) The Mre11-Rad50-Xrs2 complex is required for yeast DNA postreplication repair. PLoS One. 9: e109292. [Text]

5.           Wen, R., Wang, S., Xiang, D., Venglat, P., Shi, X., Zang, Y., Datla, R., Xiao, W. and Wang, H. (2014) UBC13, an E2 enzyme for Lys63-linked ubiquitination, functions in root development by affecting auxin signaling and Aux/IAA protein stability. Plant J.  80: 424-436. [Text]

6.           Wu, Z., Shen, S., Zhang, Z., Zhang, W. and Xiao, W. (2014) Ubiquitin-conjugating enzyme complex Uev1A-Ubc13 promotes breast cancer metastasis through nuclear factor-κB mediated matrix metalloproteinase-1 gene regulation. Breast Cancer Res. 16: R75. [Text]

7.           Cao, L., Tang, X., Zhang, X., Zhang, J., Tian, X., Wang, J., Xiong, M. and Xiao, W. (2014) Two-stage transcriptional reprogramming in Saccharomyces cerevisiae for optimizing ethanol production from xylose. Metab. Eng. 24: 150-159. [Text]

8.           Ball, L.G., Xu, X., Blackwell, S., Hanna, M.D., Lambrecht, A.D. and Xiao, W. (2014) The Rad5 helicase activity is dispensable for error-free DNA post-replication repair. DNA Repair 16: 74-83. [Text]

9.           Xu, X., Ball, L., Chen, W., Tian, X., Lambrecht, A., Hanna, M. and Xiao, W. (2013) The yeast Shu complex utilizes homologous recombination machinery for error-free lesion bypass via physical interaction with a Rad51 paralogue. PLoS One 8: e81371. [Text]

10.       Tian, X., Xu, X. and Xiao, W. (2013) Novel method for genomic promoter shuffling using recyclable cassettes. Appl. Environ. Microbiol. 79: 7042-7047. [Text]

11.       Qin, Z., Lu, M., Xu, X., Hanna, M., Shiomi, N. and Xiao, W. (2013) DNA-damage tolerance mediated by PCNA?Ub fusions in human cells is dependent on Rev1 but not Polη. Nucleic Acids Res. 41: 7356-7369. [Text]

12.       Bhat, A., Andersen, P.L., Qin, Z. and Xiao, W. (2013) The Rev3 subunit of Polζ is required for maintaining fragile site stability in human cells. Nucleic Acids Res. 41: 2328-2339. [Text]

13.       Wei, T., Zhang, C., Xu, X., Hanna, M., Zhang, X., Wang, Y., Dai, H. and Xiao, W. (2013) Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals. Biosensors and Bioelectronics. 44: 138-145. [Text]

14.       Li, L., Wang, J., Hou, J., Wu, Z., Zhuang, Y., Lu, M., Zhang, Y., Zhou, X., Li, Z., Xiao, W. and Zhang, W. (2012) Cdk1 interplays with Oct4 to repress differentiation of embryonic stem cells into trophectoderm. FEBS Lett. 586: 4100-4107. [Text]

15.       Zang, Y., Wang, Q., Xue, C., Li, M., Wen, R. and Xiao, W.  (2012) Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage. Rice 5: 24. [Text]

16.       Wen, R., Li, J., Xu, X., Cui, Z. and Xiao, W. (2012) Zebrafish Mms2 promotes K63-linked polyubiquitination and is involved in p53-mediated DNA-damage response. DNA Repair 11: 157-166. [Text]

17.       Biss, M. and Xiao, W. (2012) Selective tumor killing based on specific DNA-damage response deficiencies. Cancer Biol. & Ther. 13: 239-246. [Text]

18.       Xiang, D., Yang, H., Venglat, P., Cao, Y., Wen, R., Ren, M., Stone, S., Wang, E., Wang, H., Xiao, W., Weijers, D., Berleth, T., Laux, T., Selvaraj, G., Datla, R. (2011) POPCORN functions in the auxin pathway to regulate embryonic body plan and meristem organization in Arabidopsis. Plant Cell 23: 4348-4367. [Text]

19.       Li, J., Bhat, A. and Xiao, W. (2011) Regulation of nucleotide excision repair through ubiquitination. Acta Bioch. Biophys. Sinica 43: 919-929. [Text]

20.       Andersen, P.L., Xu, F., Ziola, B., McGregor, W.G. and Xiao, W. (2011) Sequential assembly of translesion DNA polymerases at UV-induced DNA damage sites. Mol. Biol. Cell. 22: 2373-2383. [Text]

21.       Wang, S., Wen, R., Shi, X., Lambrecht, A., Wang. H. and Xiao, W. (2011) RAD5A and REV3 constitute two alternative mechanisms of DNA damage tolerance in Arabidopsis. DNA Repair 10: 620-628. [Text]

22.       Zhang, W., Qin, Z., Zhang, X. and Xiao, W. (2011) Roles of sequential ubiquitination of PCNA in DNA-damage tolerance. FEBS Lett. 585: 2786-2794. [Text]

23.       Xu, X., Eilers, M., Xiao, W., Du, L., Burkle, A., Wiesmuller, L. and Wang, Z. (2011) The first Sino-German Symposium on DNA Repair & Human Diseases. DNA Repair 10: 349-354. [Text]

24.       Zhang, M., Zhang, C., Li, J., Hanna, M., Zhang, X., Dai, H. and Xiao, W. (2011) Inactivation of YAP1 enhances sensitivity of the yeast RNR3-lacZ genotoxicity testing system to a broad range of DNA-damaging agents. Tox. Sci. 120: 310-321. [Text]

25.       Zhang, W., Zhang, X., Liu, H., Chen, J., Ren Y., Huang, D., Zou, X. and Xiao, W. (2011) Cdk1 is required for the self-renewal of mouse embryonic stem cells. J. Cell Bioch. 112: 942-948. (Cover and featured article) [Text]

26.       Markin, C.J., Saltibus, L., Kean, M., McKay, R., Xiao, W. and Spyracopoulos, L. (2010) Catalytic proficiency of ubiquitin conjugation enzymes: balancing pKa suppression, entropy, and electrostatics. J. Am. Chem. Soc. 132: 17775-17786. [Text]

27.       Li, J., Wen, R., Andersen, P., Liang, Y., Li, Q., Xiao, W. and Cui, Z. (2010) Zebrafish Ubc13 is required for Lys63-linked polyubiquitination and DNA damage tolerance. Mol. Cell. Biochem. 343: 173-182. [Text]

28.       Power, C., Marfleet, T.W., Qualtiere, L., Xiao, W. and Bretscher, P. (2010) Development of Th1 imprints to rBCG expressing a foreign protein: implications for vaccination against HIV-1 and diverse influenza strains. J. Biomed. Biotechnol. 2010: 591348, 8 pages. [Text]

29.       Pastushok, L., Hanna, M. and Xiao, W. (2010) Constitutive fusion of ubiquitin to PCNA provides DNA damage tolerance independent of translesion polymerase activities. Nucleic Acids Res. 38: 5047-5058. [Text]

30.       Markin, C.J., Xiao, W. and Spyracopoulos, L. (2010) Mechanism for recognition of polyubiquitin chains: balancing affinity through interplay between multivalent binding and dynamics.  J. Am. Chem. Soc. 132: 11247-11258. [Text]

31.       Zhang, M., Hanna, M., Li, J., Butcher, S., Dai, H. and Xiao, W. (2010) Creation of a hyperpermeable yeast strain to genotoxic agents through combined inactivation of PDR and CWP genes. Tox. Sci. 113: 401-411. [Text]

32.       Ball, L.G., Zhang, K., Cobb, J.A., Boone, C. and Xiao, W. (2009) The yeast Shu complex couples error-free PRR to homologous recombination. Mol. Microbiol. 73: 89-102. [Text]

33.       Ball, L. and Xiao, W. (2009) Ataxia telangiectasia and its overlap with Nijmegen breakage syndrome and Ataxia-like disorders. In: Ahmad, S. (ed.) Molecular Mechanisms of Ataxia Telangiectasia. Chapter 10, pp.91-100. Landes Biosciences Publication, Texas. [Text]

34.       Pelzer, L., Pastushok, L., Moraes, T., Glover, J.N.M., Ellison, M.J., Ziola, B. and Xiao, W. (2009) Biological significance of structural differences between two highly conserved Ubc variants. Biochem. Biophy. Res. Comm. 378: 563-568. [Text]

35.       Fu, Y., Pastushok, L. and Xiao, W. (2008) DNA damage-induced gene expression in Saccharomyces cerevisiae. FEMS Microbol. Rev. 32: 908-926. [Text]

36.       Huen, M.S.Y., Yuan, J., Yamamoto, M., Akira, S., Ashley, C., Xiao, W. and Chen, J. (2008) Noncanonical E2 variant-independent function of UBC13 in promoting checkpoint protein assembly. Mol. Cell. Biol. 19: 6104-6112. [Text]

37.       Anderson, H.J., Vonarx, E.J., Pastushok, L., Nakagawa, M., Katafuchi, A., Gruz, P., Di Rubbo, A., Grice, D.M., Osmond, M.J., Sakamoto, A., Nohmi, T., Xiao, W. and Kunz, B.A. (2008) Arabidopsis thaliana Y-family DNA polymerase η catalyses translesion synthesis and interacts functionally with PCNA2. Plant J. 55: 895-908. [Text]

38.       Fu, Y., Zhu, Y. Zhang, K., Yeung, M., Durocher, D. and Xiao, W. (2008) Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clamp. Cell 133: 601-611. [Text]

39.       Zhang, M., Liang, Y., Zhang, X., Xu, Y., Dai, H. and Xiao, W. (2008) Deletion of yeast CWP genes enhances cell permeability to genotoxic agents. Toxicol. Sci. 103: 68-76. [Text]

40.       Rostek, C., Turner, E., Robbins, M., Rightnar, S., Xiao, W., Obenaus, A. and Harkness, T.A.A. (2008) Involvement of homologous recombination repair after proton-induced DNA damage. Mutagenesis 23: 119-129. [Text]

41.       Brun, J., Chiu, R.K., Lockhart, K., Xiao, W., Wouters, B.G. and Gray, D.A. (2008) hMMS2 serves a redundant role in human PCNA polyubiquitination. BMC Mol. Biol. 9: 24. [Text]

42.       Wen, R., Torres-Acosta, J.A., Pastushok, L., Lai, X., Pelzer, L., Wang, H. and Xiao, W. (2008) Arabidopsis UEV1D promotes lysine-63-linked polyubiquitination and is involved in DNA damage response. Plant Cell 20: 213-227. [Text]

43.       Andersen, P.L., Xu, F. and Xiao, W. (2008) Eukaryotic DNA damage tolerance and translesion synthesis through covalent modifications of PCNA. Cell Res. 18: 162-173. [Text]

44.       Dworaczek, H. and Xiao, W. (2007) Xeroderma pigmentosum: a glimpse into nucleotide excision repair, genetic instability, and cancer. Crit. Rev. Oncogen. 13: 159-177. (Cover and featured article) [Text]

45.       Kunz, B.A. and Xiao, W. (2007) DNA damage tolerance in plants via translesion synthesis. Genes, Genomes, Genomics 1: 89-99. [Text]

46.       Pastushok, L., Spyracopoulos, L. and Xiao, W. (2007) Two Mms2 residues cooperatively interact with ubiquitin and are critical for Lys63 polyubiquitination in vitro and in vivo. FEBS Lett. 581: 5343-5348. [Text]

47.       Hanna, M., Ball, L.G., Tong, A.H., Boone, C. and Xiao, W. (2007) Pol32 is required for Polζ-dependent translesion synthesis and prevents double-strand breaks at the replication fork. Mutat. Res. 625: 164-176. [Text]

48.       Mercier, P., Lewis, M.J., Hau, D.D., Saltibus, L.F., Xiao, W. and Spyracopoulos, L. (2007) Structure, interactions, and dynamics of the RING domain from human TRAF6. Protein Sci. 16: 602-614. [Text]

49.       Barbour, L., Ball, L.G., Zhang, K. and Xiao, W. (2006) DNA damage checkpoints are involved in postreplication repair. Genetics 174: 1789-1800. [Text]

50.       Syed, N.A., Andersen, P.L., Warrington, R.C. and Xiao, W. (2006) Uev1A, a ubiquitin conjugating enzyme variant, inhibits stress-induced apoptosis through NF-κB activation. Apoptosis 11: 2147-2157. [Text]

51.       Hau, D.D., Lewis, M.J., Saltibus, L.F., Pastushok, L., Xiao, W. and Spyracopoulos, L. (2006) Structure and interactions of the ubiquitin-conjugating enzyme variant human Uev1a: implications for enzymatic synthesis of polyubiquitin chains. Biochemistry 45: 9866-9877. [Text]

52.       Wen, R., Newton, L., Li, G., Wang, H. and Xiao, W. (2006) Arabidopsis thaliana UBC13: implication of error-free DNA damage tolerance and Lys63-linked polyubiquitylation in plants. Plant Mol. Biol. 61: 241-253. [Text]

53.       Fu, Y. and Xiao, W. (2006) Identification and characterization of CRT10 as a novel regulator of Saccharomyces cerevisiae ribonucleotide reductase genes. Nucleic Acids Res. 34: 1876-1883. [Text]

54.       Lewis, M.J., Saltibus, L.F., Hau, D.D., Xiao, W. and Spyracopoulos, L. (2006) Structural basis for non-covalent interaction between ubiquitin and the ubiquitin conjugating enzyme variant human Mms2. J. Biomol. NMR 34: 89-100. (Cover article) [Text]

55.       Barbour, L. and Xiao, W. (2006) Mating type regulation of cellular tolerance to DNA damage is specific to the DNA postreplication repair and mutagenesis pathway. Mol. Microbiol. 59: 637-650. [Text]

56.       Andersen, P., Zhou, H., Pastushok, L., Moraes, T., McKenna, S., Ziola, B., Ellison, M.J., Dixit, V.M. and Xiao, W. (2005) Distinct regulation of Ubc13 functions by two Uev proteins Mms2 and Uev1A. J. Cell Biol. 170: 745-755. [Text]

57.       Ball, L.G. and Xiao, W. (2005) Molecular basis of ataxia telangiestasia and related diseases. Acta Pharmacologica Sinica 26: 897-907. [Text]

58.       Pastushok, L., Moraes, T.F., Ellison, M.J. and Xiao, W. (2005) A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. J. Biol. Chem. 280: 17891-17900. [Text]

59.       Pastushok, L. and Xiao, W. (2004) DNA Postreplication repair modulated by ubiquitination and sumoylation. Advances in Protein Chemistry 69: 279-306. [Text]

60.       Zhu, Y and Xiao, W. (2004) Pdr3 is required for DNA damage induction of MAG1 and DDI1 via a bi-directional promoter element. Nucleic Acids Res. 32: 5066-5075. [Text]

61.       Wooff, J., Pastushok, L., Hanna, M., Yu, F. and Xiao, W. (2004) The TRAF6 RING finger domain mediates physical interaction with Ubc13. FEBS Lett. 566: 229-233. [Text]

62.       Zhou, H., Wertz, I., O'Rourke, K., Ultsch, M., Seshagiri, S., Eby, M., Xiao, W. and Dixit, V.M. (2004) Bcl10 activates the NF-κB pathway through ubiquitination of NEMO. Nature 427: 167-171. [Text]

63.       Hanna, M.D., Meadows, K.L., Chow, B.L., Jinks-Robertson, S., Doetsch, P.W. and Xiao, W. (2004) Involvement of two endonuclease III homologs in the base excision repair for the processing of DNA alkylation damage in Saccharomyces cerevisiae. DNA Repair 3: 51-59 [Text]

64.       Barbour, L. and Xiao, W. (2003) Regulation of alternative replication bypass pathways at stalled replication forks and its effects on genome stability: a yeast model. Mutat. Res. 532: 137-155. [Text]

65.       Fu, Y. and Xiao, W. (2003) Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization. DNA Repair 2: 1435-1447 [Text]

66.       Bawa, S. and Xiao, W. (2003) A single amino acid substitution in Msh5 results in DNA alkylation tolerance. Gene 315: 177-182. [Text]

67.       Jia, X. and Xiao, W. (2003) Compromised DNA repair enhances sensitivity of the RNR3-lacZ genotoxic testing system. Toxicol. Sci. 75: 82-88. [Text]

68.       McKenna, S., Hu, J., Moraes, T., Xiao, W., Ellison, M.J. and Spyracopoulos, L. (2003) Energetics and specificity of interactions within Ub.UEV.Ubc13 human ubiquitin conjugation complexes. Biochemistry 42: 7922-7930. [Text]

69.       McKenna, S., Moraes, T., Pastushok, L., Ptak, C., Xiao, W., Spyracopoulos, L. and Ellison, M.J. (2003) An NMR based model of the ubiquitin-bound human ubiquitin conjugation complex Mms2/Ubc13: The structural basis for lysine 63 chain catalysis. J. Biol. Chem. 278: 13151-13158. [Text]

70.       Brown, M., Zhu, Y., Hemmingsen, S, and Xiao, W. (2002) Structural and functional conservation of error-free postreplication repair in Schizosaccharomyces pombe. DNA Repair 1: 869-880. [Text]

71.       Jia, X., Zhu, Y. and Xiao, W. (2002) A stable and sensitive genotoxic testing system based on DNA damage induced gene expression in Saccharomyces cerevisiae. Mutat. Res. 519: 83-92. [Text]

72.       Li, Z., Xiao, W., McCormick, J.J. and Maher, V.M. (2002) Identification of a protein essential for a major pathway used by human cells to avoid UV-induced DNA damage. Proc. Natl. Acad. Sci. USA 99: 4459-4464. [Text]

73.       Ashley, C., Pastushok, L., McKenna, S., Ellison, M.J. and Xiao, W. (2002) Roles of mouse UBC13 in DNA postreplication repair and Lys63-linked ubiquitination. Gene 285: 183-191. [Text]

74.       Broomfield, S. and Xiao, W. (2002) Suppression of genetic defects within the RAD6 pathway by srs2 is specific for error-free postreplication repair but not for damage induced mutagenesis. Nucleic Acids Res. 30: 732-739. [Text]

75.       Hryciw, T., Tang, M., Fontanie, T. and Xiao, W. (2002) MMS1 protects against replication-dependent DNA damage in Saccharomyces cerevisiae. Mol. Genet. Genomics 266: 848-857. [Text]

76.       Villalobo, E., Morin, L., Moch, C., Lescasse, R., Hanna, M., Xiao, W. and Baroin-Tourancheau, A. (2002) A homolog of CROC-1 in a ciliated protist (Sterkiella histriomuscorum) testifies to the ancient origin of the ubiquitin-conjugating enzyme variant family. Mol. Biol. Evol. 19: 39-48. [Text]

77.       Xiao, W., Chow, B.L., Hanna, M. and Doetsch, P.W. (2001) Deletion of the MAG1 glycosylase gene suppresses alkylation-induced killing and mutagenesis in yeast cells lacking AP endonucleases. Mutat. Res. 487: 137-147. [Text]

78.       McKenna, S., Spyracopoulos, L., Moraes, T., Pastushok, L., Ptak, C., Xiao, W. and Ellison, M.J. (2001) Non-covalent interaction between ubiquitin and the human DNA repair protein Mms2 is required for Ubc13-mediated poly-ubiquitination. J. Biol. Chem. 276: 40120-40126. [Text]

79.       Zhu, Y. and Xiao, W. (2001) Two alternative cell cycle checkpoint pathways differentially control DNA damage induction of the yeast MAG1 and DDI1 expression. Mol. Genet. Genomics 266: 436-444. [Text]

80.       Moraes, T.F., Edwards, R.A., McKenna, S., Pastushok, L., Xiao, W., Glover, J.N.M. and Ellison, M.J. (2001) Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13. Nature Structural Biol. 8: 669-673.  (Cover article) [Text]

81.       Broomfield, S., Hryciw, T. and Xiao, W. (2001) DNA postreplication repair and mutagenesis in Saccharomyces cerevisiae. Mutat. Res. 486: 167-184. [Text]

82.       Dai, H., Zhao, R., Haug, B., Hemmingsen, S.M. and Xiao, W. (2001) Isolation by phage display and characterization of a single-chain antibody specific for O6-methyldeoxyguanosine. Chinese Science Bulletin 46: 1024-1029. [Text]

83.       Franko, J., Ashley, C. and Xiao, W. (2001) Molecular cloning and functional characterization of two murine cDNAs encoding Ubc-like proteins involved in DNA repair and mutagenesis. Biochem. Biophys. Acta. 1519: 70-77. [Text]

84.       Barbour, L., Zhu, Y. and Xiao, W. (2000) Improving synthetic lethal screens by regulating the yeast centromere sequence. Genome 43: 910-917. [Text]

85.       Xiao, W., Chow, B.L., Broomfield, S. and Hanna, M. (2000) The Saccharomyces cerevisiae RAD6 group is composed of an error-prone and two error-free postreplication repair pathways. Genetics 155: 1633-1641. [Text]

86.       Brusky, J., Zhu, Y. and Xiao, W, (2000) UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae. Curr. Genet. 37: 168-174. [Text]

87.       Chamankhah, M., Fontanie, T. and Xiao, W. (2000) The Saccharomyces cerevisiae mre11(ts) allele confers a separation of DNA repair and telomere maintenance functions. Genetics 155: 569-576. [Text]

88.       Xiao, W., Fontanie, T., Bawa, S. and Kohalmi, L. (1999) REV3 is required for spontaneous but not methylation damage-induced mutagenesis of Saccharomyces cerevisiae cells lacking O6-methylguanine DNA methyltransferase. Mutat Res. 431: 155-165. [Text]

89.       Bawa S. and Xiao, W. (1999) Methionine reduces spontaneous and alkylation-induced mutagenesis in Saccharomyces cerevisiae cells deficient in O6-methylguanine-DNA methyltransferase. Mutat Res. 430: 99-107. [Text]

90.       Xiao, W,. Chow, B.L., Fontanie, T., Ma, L., Bacchetti, S., Hryciw, T. and Broomfield, S. (1999) Genetic interactions between error-prone and error-free postreplication repair pathways in Saccharomyces cerevisiae. Mutat. Res. 431: 155-165. [Text]

91.       Chamankhah, M. and Xiao, W. (1999) Formation of the yeast Mre11-Rad50-Xrs2 complex is correlated with DNA repair and telomere maintenance. Nucleic Acids Res. 27: 2072-2079. [Text]

92.       Chamankhah, M., Wei, Y.-F. and Xiao, W. (1998) Isolation of hMRE11B:failure to complement yeast mre11 defects due to species-specific protein interactions. Gene 225: 107-116. [Text]

93.       Chamankhah, M. and Xiao, W. (1998) Molecular cloning and genetic characterization of the Saccharomyces cerevisiae NGS1/MRE11. Current Genet. 34: 368-374. [Text]

94.       Zhu, Y. and Xiao, W. (1998) Differential regulation of two closely clustered yeast genes, MAG1 and DDI1, by cell-cycle checkpoints. Nucleic Acids Res. 26: 5402-5408. [Text]

95.       Kunz, B.A., Henson, E.S., Karthikeyan, R., Kuschak, T., McQueen, S.A., Scott, C.A. and Xiao, W. (1998) Defects in base excisioin repair combined with elevated intracellular dCTP levels dramatically reduce mutation induction in yeast by ethyl methanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine. Envir. Mol. Mutag. 32: 173-178. [Text]

96.       Ma, L., Broomfield, S., Lavery, C., Lin, S., Xiao, W. and Bacchetti, S. (1998) Up-regulation of CIR1/CROC-1 expression upon cell immortalization and in tumour-derived human cell lines. Oncogene 17: 1321-1326. [Text]

97.       Xiao, W., Lin, S.L., Broomfield, S., Chow, B.L. and Wei, Y.F. (1998) The products of the yeast MMS2 and two human homologs (hMMS2 and CROC-1) define a structurally and functionally conserved Ubc-like protein family. Nucleic Acids Res. 26: 3908-3914 [Text]

98.       Xiao, W., Lechler, T., Chow, B.L., Fontanie, T., Agustus, M., Carter, K.C. and Wei, Y.-F. (1998) Identification, chromosomal mapping and tissue-specific espression of hREV3 encoding a putative human DNA polymerase ζ. Carcinogenesis 19: 945-949. [Text]

99.       Xiao, W., Chow, B.L. and Milo, C.N. (1998) Mms4, a putative transcriptional (co)activator, protects Saccharomyces cerevisiae cells from endogenous and environmental DNA damage. Mol. Gen. Genet. 257: 614-623. [Text]

100.   Xiao, W. and Chow, B.L. (1998) Synergism between yeast nucleotide and base excision repair pathways in the protection against DNA methylation damage. Curr. Genet. 33: 92-99. [Text]

101.   Broomfield, S., Chow, B.L. and Xiao, W. (1998) MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway. Proc. Natl. Acad. Sci. USA 95: 5678-2683. [Text]

102.   Bawa, S. and Xiao, W. (1997) A mutation in the MSH5 gene results in alkylation tolerance. Cancer Res. 57: 2715-2720. [Text]

103.   Liu, Y., Dai, H. and Xiao, W. (1997) UASMAG1, a yeast cis-acting element that regulates the expression of MAG1, is located within the protein coding region of DDI1. Mol. Gen. Genet. 255: 533-542. [Text]

104.   Liu, Y. and Xiao, W. (1997) Bidirectional regulation of two DNA-damage-inducible genes, MAG1 and DDI1, from Saccharomyces cerevisiae. Mol. Microbiol. 23: 777-789. [Text]

105.   Xiao, W., Chow, B.L. and Rathgeber, L. (1996) The repair of DNA methylation damage in Saccharomyces cerevisiae. Curr. Genet. 30: 461-468. [Text]

106.   Xiao, W. and Rank, G.H. (1996) The 2μm plasmid of laboratory yeast strains is a type-1/type-2 hybrid. Yeast 12: 809-813. [Text]

107.   Xiao, W., Nowak, M., Laferte, S. and Fontanie, T. (1996) Mutagenicity and toxicity of the DNA alkylation carcinogens 1,2-dimethylhydrazine and azoxymethane in Escherichia coli and Salmonella typhimurium. Mutagenesis 11: 241-245. [Text]

108.   Xiao, W., Rathgeber, L., Fontanie, T. and Bawa, S. (1995) DNA mismatch repair mutants do not increase N-methyl-N'-nitro-N-nitrosoguanidine tolerance in O6-methylguanine DNA methyltransferase-deficient yeast cells. Carcinogenesis 16: 1933-1939. [Text]

109.   Xiao, W. and Fontanie, T. (1995) Expression of the human MGMT O6-methylguanine DNA methyltransferase gene in a yeast alkylation-sensitive mutant: its effects on both exogenous and endogenous DNA alkylation damage. Mutat. Res. 336: 133-142. [Text]

110.   Xiao, W., Fontanie, T. and Tang, M. (1994) UBP5 encodes a putative yeast ubiquitin-specific protease that is related to the human Tre-2 oncogene product. Yeast 10: 1497-1502. [Text]

111.   Rank, G.H., Xiao, W. and Pelcher, L.E. (1994) Transpogenes: the transposition-like integration of short sequence DNA into the yeast plasmid creates the STB locus and plasmid-size polymorphism. Gene 147: 55-61. [Text]

112.   Xiao, W., Penugonde, V. and Rank, G.H. (1994) The MAG1-3-methyladenine DNA glycosylase gene is closely linked to the SPT15 TATA-binding TFIID gene on chromosome V-R in Saccharomyces cerevisiae. Yeast 10: 687-691. [Text]

113.   Rank, G.H., Xiao, W. and Arndt, G.M. (1994) Evidence for Darwinian selection of the 2μm plasmid STB locus in Saccharomyces cerevisiae. Genome 37: 12-18. [Text]

114.   Xiao, W., Singh, K.K., Chen, B., Samson, L. (1993) A common element involved in transcriptional regulation of two DNA alkylation repair genes (MAG and MGT1) of Saccharomyces cerevisiae. Mol. Cell. Biol. 13: 7213-7221. [Text]

115.   Xiao, W. and Samson, L. (1993) In vivo evidence for endogenous DNA alkylation damage as a source of spontaneous mutation in eukaryotic cells. Proc. Natl. Acad. Sci. USA 90: 2117-2121. [Text]

116.   Xiao, W. and Rank, G.H. (1993) Polymorphism of 2μm plasmids in industrial strains of Saccharomyces cerevisiae. Appl. Microbiol. & Biotechnol. 39: 80-86. [Text]

117.   Xiao, W. and Samson, L. (1992) The Saccharomyces cerevisiae MGT1 DNA repair methyltransferase gene: its promoter and entire coding sequence, regulation and in vivo biological functions. Nucleic Acids Res. 20: 3599-3606. [Text]

118.   Xiao, W., Derfler, B., Chen, J. and Samson, L. (1991) Primary sequence and biological functions of a Saccharomyces cerevisiae O6-methylguanine/O4-methylthymine DNA repair methyltransferase gene. EMBO J. 10: 2179-2186. [Text]

119.   Rank, G.H. and Xiao, W. (1991) Alteration of industrial food and beverage yeasts by recombinant DNA technology. Ann. New York Acad. Sci. 646: 155-171.

120.   Rank, G.H., Casey, G.P., Xiao, W. and Pringle, A.T. (1991) Polymorphism within the nuclear and 2μm genomes of Saccharomyces cerevisiae. Curr. Genet. 20: 189-194. [Text]

121.   Xiao, W., Pelcher, L.E. and Rank, G.H. (1991) Sequence diversity of yeast 2μm RAF gene and its coevolution with STB and REP1. Gene 101: 75-80. [Text]

122.   Xiao, W., Pelcher, L.E. and Rank, G.H. (1991) DNA sequence divergence and functional conservation at the STB locus of yeast 2μm circle variants. J. Bacteriol. 171: 1181-1186. []

123.   Xiao, W., Pelcher, L.E. and Rank, G.H. (1991) Evidence for cis- and trans-acting element coevolution of the 2μm circle genome in Saccharomyces cerevisiae. J. Mol. Evol. 32: 145-152. [Text]

124.   Xiao, W., and Rank, G.H. (1990) Curing industrial Saccharomyces yeasts of parasitic 2μm plasmid. J. Am. Soc. Brew. Chem. 48: 107-110.

125.   Xiao, W. and Rank, G.H. (1990) Branched chain amino acid regulation of the ILV2 locus in Saccharomyces cerevisiae. Genome 33: 596-603. [Text]

126.   Xiao, W. and Rank, G.H. (1990) Cloning of industrial Saccharomyces 2μm plasmid variants by in vivo site-specific recombination. Plasmid 23: 67-70. [Text]

127.   Xiao, W. and Rank, G.H. (1990) An improved method for yeast 2μm plasmid curing. Gene 88: 241-245. [Text]

128.   Xiao, W. and Rank, G.H. (1989) The construction of recombinant industrial yeasts free of bacterial sequences by direct gene replacement into a nonessential region of the genome. Gene 76: 99-107. [Text]

129.   Rank, G.H., Arndt, G. and Xiao, W. (1989) FLP-FRT mediated intra-chromosomal recombination on a tandemly duplicated YEp integrant at the ILV2 locus of chromosome XIII in S. cerevisiae. Curr Genet. 15: 107-112. [Text]

130.   Xiao, W. and Rank, G.H. (1988) Generation of an ilv bradytrophic phenocopy in yeast by antisense RNA. Curr. Genet. 13: 283-289.

131.   Xiao, W. and Rank, G.H. (1988) The yeast ILV2 gene is under general amino acid control. Genome 30: 984-986. [Text]

132.   Rank, G.H., Casey, G. and Xiao, W. (1988) Gene transfer in industrial Saccharomyces yeasts. Food Biotechnol. 2: 1-41.

133.   Xiao, W. (1988) Stability of cloned Brassica napus chloroplast DNA fragments in the cyanobacterium Anacystis nidulans R2. Mol. Gen. Genet. 214: 307-312.

134.   Casey, G.P., Xiao, W. and Rank, G.H. (1988) Construction of α-galactosidase-positive strains of industrial baker's (Saccharomyces cerevisiae) yeasts. J. Am. Soc. Brew. Chem. 46: 67-71.

135.   Casey, G.P., Xiao, W. and Rank, G.H. (1988) Application of pulsed field chromosome electrophoresis in the study of chromosome XIII and the electrophoretic karyotype of industrial strains of Saccharomyces yeasts. J. Inst. Brew. 94: 239-243.

136.   Rank, G.H., Xiao, W., Kolenovsky, A. and Arndt, G. (1988) FLP recombinase induction of the breakage-fusion-bridge cycle and gene conversion in Saccharomyces cerevisiae. Curr. Genet. 13: 273-281.

137.   Casey, G.P., Xiao, W. and Rank, G.H. (1988) A convenient dominant selection marker for gene transfer in industrial strains of Saccharomyces yeast: SMR1 encoded resistance to the herbicide sulfometuron methyl. J. Inst. Brew. 94: 93-97.

138.   Xiao, W., Saxena, P.K., King, J. and Rank, G.H. (1987) A transient duplication of the acetolactate synthase gene in a cell culture of Datura innoxia. Theor. Appl. Genet. 74: 417-422.

139.   Xiao, W., Reith, M., Erickson, L.R., Williams, J.P. and Straus, N.A. (1986) Mapping the chloroplast genome of triazine resistant canola. Theor. Appl. Genet. 71: 716-723.