李夏璐

作者:生命科学院 | 来源: | 发布日期:2013-04-09 | 阅读次数:

李夏璐,博士生导师,教授
电话:010-68901794
邮箱: lixialu_cnu@163.com

硕士招生: 细胞生物学 肿瘤细胞基因组不稳定性

博士招生:生物化学与分子生物学  肿瘤细胞基因组不稳定性和真核细胞mRNA加工

教育经历
2000 年 中国科学院上海生物化学研究所分子生物学博士
1995 年 复旦大学生物化学系生物化学学士


工作经历
2012 年12 月- 首都师范大学特聘教授
2006 年-2012 年12 月 北京生命科学研究所研究员
2005-2006 年 美国哥伦比亚大学生物系助理研究员
2000-2005 年 美国哥伦比亚大学生物系博士后


研究领域
1. 基因组稳定性
2. 基因表达调控
3. DNA 复制
4. RNA 加工


研究概述
RNA转录和DNA复制是生命过程中必须发生的两个核心生物学事件。然而,我们前期的研究工
作意外地揭示,无时无刻进行着的RNA转录可以通过形成R-loop干扰DNA复制叉运行, 进而影
响基因组稳定性。维持基因组稳定是生命得以存在和延续的根本。毫无疑问,机体必然相应
地发展形成一套有效的应对机制以维护基因组稳定。本实验室的主要研究兴趣在于运用遗传
学,分子生物学,生物化学和生物信息学等方法,理解哺乳动物细胞是如何应对在生长和增
殖过程中似乎是必然会出现的转录、复制和基因组稳定性,以维护三者间的冲突;并探索这
一冲突形成后可能产生的病理后果。希望我们的研究能有助于加深对于肿瘤等重大疾病发病
机理的理解,从而为癌症的诊断、治疗和药物的开发提供新的理论基础.


目前实验室正在开展的工作包括:
1. R-loop破坏基因组不稳定性的应对机制
2. 转录在肿瘤发生以及肿瘤细胞染色体不稳定性建立过程中的作用
3. 染色体脆性表达的分子机理
4. 哺乳动物细胞中停顿复制叉的稳定和重新启动的分子机制
5. 人基因组DNA复制起始的时、空调控机制
6. RNA加工过程在癌症发生和发展中的作用
欢迎对上述科学问题感兴趣的同学加入本实验室(接受调剂生)。
 

发表文章 (引文数据来自Google Scholar 查询结果
1. Yi Wei, Fan Yang, Chuanli Tuju, Jie Liu, Yonggong Zhai and X. Li *, Molecular
basis for the expression of chromosome fragility(*corresponding author) (in
preparation)
2. B. Liang, P. Peng, S. Chen, L. Lin, D. Cao, J. Yang, H. Li, T. Gui, X. Li* and
K. Shen*Characterization and proteomic analysis of ovarian cancer-derived
exosomes, Journal of Proteomics, 80 (2013) 171-182 (*corresponding author)
3. W. Jin, Z. Niu, D. Xu and X. Li*, RBM5 promotes exon 4 skipping of AID pre-mRNA
by competing with the binding of U2AF65 to the polypyrimidine tract, FEBS Lett
586 (2012) 3852-3857. (*corresponding author)
4. Z. Niu, W. Jin, L. Zhang, X. Li*, Tumor suppressor RBM5 directly interacts with
the DExD/H-box protein DHX15 and stimulates its helicase activity, FEBS Lett
586 (2012) 977-983. (*corresponding author) (times cited: 3)
5. Y. Kanehiro, K. Todo, M. Negishi, J. Fukuoka, W. Gan, T. Hikasa, Y. Kaga, M.
Takemoto, M. Magari, X. Li, J.L. Manley, H. Ohmori, N. Kanayama,
Activation-induced cytidine deaminase (AID)-dependent somatic hypermutation
requires a splice isoform of the serine/arginine-rich (SR) protein SRSF1, Proc
Natl Acad Sci USA 109 (2012) 1216-1221. (Selected by faculty 1000 of biology)
(times cited: 5)
6. W. Gan, Z. Guan, J. Liu, T. Gui, K. Shen, J.L. Manley, X. Li*, R-loop-mediated
genomic instability is caused by impairment of replication fork progression,
Genes & Development, 25 (2011) 2041-2056. (*corresponding author) (Selected by
faculty 1000 of biology) (times cited: 19)
7. X. Li and James L. Manley, 2009, The Role of Alternative Splicing During the
Cell Cycle and Programmed Cell Death. In Ralph A. Bradshaw and Edward A. Dennis,
editors: Handbook of Cell Signaling 2nd edition, Oxford:Academic Press, , pp.
2329-2334. (invited book chapter)
8. X. Li *, Tianhui Niu., and James L Manley. 2007. The RNA binding protein RNPS1
alleviates ASF/SF2 depletion-induced genomic instability. RNA 13(12): 2108-2115
(*corresponding author). (times cited: 11)
9. X. Li and James L Manley, 2006, Co-transcriptional processes and their influence
on genome stability. Genes & Development, 2006; 20(14):1838-1847. (times cited:
63)
10. X. Li and James L Manley. 2006. Alternative Splicing and Control of Apoptotic
DNA Fragmentation. Cell Cycle 5(12).1286-1288. (times cited: 5)
11. X. Li and James L. Manley, 2005, New talents for an old acquaintance: the SR
protein splicing factor ASF/SF2 functions in the maintenance of genome stability.
Cell cycle, 4(12): 1706-1708. (times cited: 19)
12. X. Li, Jin Wang and James L Manley, 2005, Loss of splicing factor ASF/SF2 induces
G2 cell-cycle arrest and apoptosis, but inhibits internucleosomal DNA
fragmentation, Genes & Development, 19(22):2705-2714. (times cited: 76)
13. X. Li and James L Manley, 2005, Inactivation of the SR protein splicing factor
ASF/SF2 results in genomic instability. Cell, 122 (3), p365-378. (Selected by
faculty 1000 of biology) (times cited: 214)
14. Boliang Li, X. Li et al., 1999, Human acyl-CoA:Cholesterol acyltransferase-1
(ACAT-1) gene organization and evidence that the 4.3-kilobase ACAT-1 mRNA is
produced from two different chromosomes, J Biol Chem, 274 (16):
p11060-11071.(times cited: 109)

 

获奖情况:
1. 1999 年中国科学院院长特别奖
2. 1999 年中国科学院地奥一等奖