李静

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李静 博士

首都师范大学教授,博士生导师

Jing Li, Ph.D.
Professor, Capital Normal University, Beijing, China

Phone:010-68901692
E-mail: jing_li@ mail.cnu.edu.cn

招生方向:细胞

研究方向:蛋白质修饰


教育经历

Education

2006      耶鲁大学分子、细胞及发育生物学系,博士

       (导师:G. Shirleen Roeder, 美国科学院院士)

       Ph. D., Molecular, Cellular and Developmental Biology, Yale University

2001      耶鲁大学分子、细胞及发育生物学系,硕士

       M. S., Molecular, Cellular and Developmental Biology, Yale University

1999      北京大学生命科学学院,学士

       B. S., College of Life Sciences, Peking University, China

荣誉

Awards

2020         细胞生物学-首批国家级一流本科课程

2019         细胞生物学-首批北京市优质本科课程

2019         首批北京市高校优秀专业课主讲教师

2019         首都师范大学本科生毕业论文(设计)优秀指导教师

2017       北京市首届高等学校青年教学名师

2017       首都师范大学优秀教师

2017       首都师范大学最受学生喜爱导师,十大影响力师门

2016         北京市教委市属高校“创想杯”多媒体课件制作与微课程应用大奖赛三等奖

2015       教育部全国高校教师网络培训中心主办全国高校微课教学比赛 教学设计奖 作品:《细胞周期》

2015-2018      首都师范大学优秀主讲教师

2012       北京市高等教育教学成果奖一等奖(排名第三)

2011       首都师范大学首届青年教师优秀教学奖

2011       首都师范大学2011届本科生毕业论文(设计)优秀指导教师

2011       第七届北京市青年教师教学基本功比赛二等奖

2010       霍英东教育基金会第十二届高等院校青年教师奖三等奖

2009       首都师范大学青年教师教学基本功竞赛一等奖,最佳教案奖

2007       北京市科技新星

1999-2006      耶鲁大学━香港范氏奖学金 (Fan Fellowship)

1998         北京大学光华奖奖学金

工作经历

Professional Experience

2018.1-今        首都师范大学教授

          Professor, CNU

2019.1-今       首都师范大学生物科学专业负责人

2009.1-2019.12     首都师范大学生科院细胞生物学教学团队队长

          Director, Cell Biology Teaching Group, CNU

2008.9-2012.12     首都师范大学生科院院长助理

          Dean Assistant, College of Life Sciences, CNU                

2008.1-2017.12     首都师范大学副教授

          Associate Professor, CNU

2007.5-2007.12     首都师范大学讲师

          Assistant Professor, CNU

2006.4-2006.7      耶鲁大学分子、细胞及发育生物学系,博士后

          Postdoctoral Associate, MCDB, Yale University

学术兼职

2021-       中国生物物理学会糖生物学分会委员会委员

2021        国自然优青函评专家,教育部全国优秀教材函评专家,国家级一流本科课程函评专家

2020      国自然生命科学部交叉融合科学处函评专家

2020-       上海嘉会医疗,顾问

2019-       苏州生物医药创新中心,学术委员会委员

2019        国自然生物二处、交叉融合科学处函评专家

2017-今    《中国生物化学与分子生物学报》英文编辑

2017      国自然生物三处函评专家

2016      美国阿尔茨海默研究基金Alzheimer’s Association Research Grant (AARG)

              函评专家

2015-2020    《遗传》英文润色编辑

2013.10     北京市科学技术研究院青年骨干评审专家

2013      教育部“留学回国人员科研启动基金”评审专家

2012      国自然生物五处函评专家

参与审稿的学术期刊

Nature Chemical Biology, Nucleic Acids Research, Frontiers in Pharmacoloy, Journal of Genetics and Genomics, Pharmaceuticals, Frontiers in Oncology, Frontiers in Genetics, Cell Cycle, Scientific Reports, Cancer Medicine, Medical Research Archives, Journal of Cancer Biology & Research,《遗传》,《中国生物化学与分子生物学报》


教材

1.李静,印莉萍,于荣, 《细胞生物学数字课程》, 高等教育出版社,2019,ISBN:978-7-89510-559-1

2.印莉萍,李静,于荣,《细胞生物学实验技术教程》(第四版),科学出版社,2015, ISBN:978-7-03-044357-1

3.许兴智,佟伟民,李静,杨跃,丛羽生, 《癌症的分子基础》, 科学出版社,2011,978-7-0-303160-7


研究概述

蛋白质的翻译后修饰(包括磷酸化、泛素化以及O-GlcNAc糖基化)参与有丝分裂后期染色体分离及胞质分离过程以及DNA 损伤检验点的机制研究,及其与人类疾病的关系。

Research Description

Mechanistic studies of protein post translational modifications (PTMs), e.g. phosphorylation, ubiquitination and O-GlcNAc glycosylation, involved in the process of chromosome segregation and cytokinesis during unperturbed cell cycle, as well as in the DNA damage repair network (DDR), with particular emphasis on its relationship with human diseases.

基金情况

Funding

时间

项目来源及编号

名称

资助金额

本人承担任务

2007.10-2010.10

北京市科委科技新星

2007B062

异源核蛋白K在抗癌的DNA损伤检验点中的功能分析

28万元

课题负责人(PI

2008.1 -2010.12

国家自然基金委青年项目30700420

DNA损伤检验点激酶Chk2的功能分析

20万元

课题负责人(PI

2008.1 -2010.12

北京市教委面上项目

KM200810028013

异源核蛋白K在抗癌的DNA损伤检验点中的功能分析

15万元

课题负责人(PI

2008.10-2009.9

北京市留学人员科技活动择优项目资助经费

DNA损伤检验点激酶Chk2的功能分析

7万元

课题负责人(PI

2008.10-2009.9

教育部留学回国人员资助

DNA损伤检验点激酶Chk2的功能分析

2.5万元

课题负责人(PI

2012.1-2016.12

国家自然科学基金重点项目31130017

参与DNA双链断裂修复的蛋白磷酸酶的功能鉴定

290万元

参与

2014.1-2016.12

北京市属高等学校青年拔尖人才培育计划

CIT&TCD201404158

Aurora B调控Ataxin-10Plk1的相互作用参与胞质分离

30万元

课题负责人(PI

2015.1-2017.12

首师大青年燕京学者培育计划

青年燕京学者培育计划

20万元

课题负责人(PI

2016.1-2017.12

北京市科委科技新星交叉课题 (Z161100004916042)

东亚人特异的年龄相关性黄斑变性基因UBE3D与细胞周期的关系及潜在的抑制剂开发

25万元

课题负责人(PI

2018.1-2018.12

北京市教委财政科研类专项交叉科研项目

(025185305000)

FEVR1参与中国人视网膜病的机理及基因治疗研发

7.7万元

课题负责人(PI

2018.1-201812

北京市高等学校青年教学名师

青年教学名师

10万元

课题负责人(PI

2019.1-2019.12

北京市教委财政科研类专项交叉科研项目(19530050137)

PCV1调控中国人脉络膜血管病变的机理研究

10万元

课题负责人(PI

2019.1-2021.12

首师大青年燕京学者计划

青年燕京学者计划

30万元

课题负责人(PI

2019.1-2022.12

国家自然基金委面上项目

31872720

ULK1O-GlcNAc修饰调控高葡萄糖诱导的自噬的机制研究

59万元

课题负责人(PI

Publications (Corresponding *)(第一/通讯18篇,其中Nature Index 4)


  1. 1. Ningda Xu, Jiarui Li, Tianchang Tao, Caifei Liu, Yan Shen, Wei Du, Li Zhu, Xin Tang, Yi Cai, Xuan Shi, Xiao-Xin Li, Leiliang Zhang, Jing Li* and Lvzhen Huang* 2021. A novel variant of SNX31 contributes to autosomal dominant familial exudative vitreoretinopathy (FEVR) pathogenesis. Frontiers in Genetics, revision


  2. 2.Yue Liu1, Ningda Xu1, Shanshan Nai1, Yong Tao1, Yuehe Ding, Lemei Jia, Qizhi Geng, Yujing Bai, Gong-Hong Wei, Meng-Qiu Dong, Xingzhi Xu, Xiao-Xin Li*, Lvzhen Huang*, Jing Li*. 2021. Age-related Macular Degeneration (AMD)-associated UBE3D interacts with KAP1 and regulates double-strand break repair. submitted


  3. 3. Yan-Fang Chen, Guang-Can Shao, Jie Li, An-Qi Yang, Jing Li, and Xin-Shan Ye* 2021 O-GlcNAcylation of Blimp-1 in lymphocytes inhites its transcriptional function and is associated with breast cancer metastasis. Molecular Cancer Research.revision


  4. 4. Jing-Jing Zhu1, Yanfang Chen1, Jing Li, and Xin-Shan Ye*. 2021 O-GlcNAcylation increases PYGL activity by promoting phosphorylation. Glycobiology, revision


  5. 5.Aiyun Yuan1, Xiangyan Tang1 and Jing Li* 2021 Centrosomes: Til O-GlcNAc do us apart. Frontiers in Endocrinology 11:621888


  6. 6.李静*,谭忠平 2020 葡糖酰胺(O-GlcNAc)修饰:细胞内糖基化的前世今生 《中国生物化学与分子生物学报》 (特约综述)36(9): 987-1001


  7. 7. Bo Ma, Xiaoyang Guan, Yaohao Li, Shiying Shang, Jing Li* and Zhongping Tan*. 2020 Protein glycoengineering:An approach for improving protein properties. Frontiers in Chemistry. 8:622


  8. 8. Caifei Liu1, Yingxin Shi1, Jie Li1, Xuewen Liu, Zhikai Xiahou, Zhongping Tan, Xing Chen and Jing Li*. 2020 O-GlcNAcylation of myosin phosphatase targeting subunit 1 (MYPT1) dictates timely disjunction of centrosomes. Journal of Biological Chemistry. 295(21):7341-7349


  9. 9. Fu-Cheng Wang, Bin Peng, Sheng-Li Cao, Hong-Yun Li, Xiao-Li Yuan, Ting-Ting Zhang, Ruifeng Shi, Zhuqing Li, Ji Liao, Hailong Wang, Jing Li and Xingzhi Xu*. 2020 Synthesis and cytotoxic activity of chalcone analogues containing a thieno[2,3-d]pyrimidin-2-yl group as the A-ring or B-ring. Bioorganic Chemistry 10:103346


  10. 10. Shanshan Nai1, Yingxin Shi1, Huanwei Ru1, Yuehe Ding, Qizhi Geng, Zhe Li, Meng-Qiu Dong, Xingzhi Xu and Jing Li* 2019 Chk2-dependent phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) regulates centrosome maturation. Cell Cycle 18(20):2651-2659



  11. 11. Xue Han1, Bin Peng1, Bei-Bei Xiao1, S.-Li Cao*, Chao-Rui Yang, Wen-Zhu Wang, Fu-Cheng Wang, Hong-Yun Li, Xiao-Li Yuan, Ruifeng Shi, Ji Liao, Hailong Wang, Jing Li, Xingzhi Xu*. 2019 Synthesis and evaluation of chalcone analogues containing a 4-oxoquinazolin-2-yl group as potential anti-tumor agents. European Journal of Medicinal Chemistry. 162:586-601




  12. 12.  Caifei Liu and Jing Li* 2018 O-GlcNAc: a sweetheart of the cell cycle and DNA damage response. Frontiers in Endocrinology (Invited) 9:415.




  13. 13.  Xiaomei Hu1, Zhe Li1, Yuehe Ding, Qizhi Geng, Zhikai Xiahou, Huanwei Ru, Meng-Qiu Dong, Xingzhi Xu and Jing Li*. 2018 Chk1 modulates the interaction between myosin phosphatase targeting protein 1 (MYPT1) and protein phosphatase 1cβ (PP1cβ). Cell Cycle. 17 (4): 421–427




  14. 14.  Chao-rui Yang1, Bin Peng1, Sheng-Li Cao*, Ting-ting Ren, Wei Jiang, Fu-Cheng Wang, You-Shan Li, Guo Wang, Zheng Li, Shibin Xu, Ji Liao, Hailong Wang, Jing Li, Xingzhi Xu*. 2018. Synthesis, cytotoxic evaluation and target identification of thieno[2,3-d]pyrimidine derivatives with a dithiocarbamate side chain at C2 position. European Journal of Medicinal Chemistry, 154: 324-340




  15. 15.  Xiaolu Ma1, Hongmei Liu1, Jing Li1,Yihao Wang, Yuehe Ding, Hongyan Shen, Yeran Yang, Chenyi Sun,Min Huang, Yingfeng Tu,Yang Liu,Yongliang Zhao, Mengqiu Dong, Ping Xu, Tie-Shan Tang*, Caixia Guo* 2017 Polη O-GlcNAcylation governs genome integrity during translesion DNA synthesis. Nature Communications. 8(1):1941. (1 Equal Contributions)




  16. 16.  Jie Tian1, Yingxing Shi1, Shanshan Nai, Qizhi Geng, Lei-Liang Zhang, Gong-Hong Wei, Xingzhi Xu* and Jing Li*. 2017. Ataxin-10 is involved in Golgi membrane dynamics. Journal of Genetics and Genomics. 44(11):549-552




  17. 17.  Zhe Li1,Xueyan Li1,Shanshan Nai, Qizhi Geng, Ji Liao, Xingzhi Xu* and Jing Li*. 2017 Checkpoint kinase 1-induced phosphorylation of O-Linked β-N-Acetylglucosamine transferase regulates the intermediate filament network during cytokinesis. Journal of Biological Chemistry. 292(48) 19548–19555




  18. 18.  Xueyan Li, Shanshan Nai, Yuehe Ding, Qizhi Geng, Bingtao Zhu, Kai Yu, Wei-Guo Zhu, Meng-Qiu Dong, Xiao-Dong Su, Xingzhi Xu* and Jing Li*. 2017 Polo-like kinase 1 (PLK1)-dependent phosphorylation of methylenetetrahydrofolate reductase (MTHFR) regulates replication via histone methylation. Cell Cycle 16(20):1933-1942




  19. 19.  Li YS, Peng B, Ma L, Cao SL, Bai LL, Yang CR, Wan CQ, Yan HJ, Ding PP, Li ZF, Liao J, Meng YY, Wang HL, Li J, Xu X. 2017 Synthesis, crystal structures and antitumor activity of two platinum(II) complexes with methyl hydrazinecarbodithioate derivatives of indolin-2-one. Eur J Med Chem. 127:137-146.




  20. 20.  Jing Li* and Xingzhi Xu*. 2016 DNA double-strand break repair: a tale of pathway choices. Acta Biochimica et Biophysica Sinica. 48(7):641-6.




  21. 21.  Jie Tian1, Qizhi Geng1, Yuehe Ding, Ji Liao, Meng-Qiu Dong, Xingzhi Xu* and Jing Li*. 2016 O-GlcNAcylation antagonizes phosphorylation of Cdh1 (CDC20 homologue 1). Journal of Biological Chemistry. 291(23):12136-44.




  22. 22.  Ding PP, Gao M, Mao BB, Cao SL, Liu CH, Yang CR, Li ZF, Liao J, Zhao H, Li Z, Li J, Wang H, Xu X. 2016.Synthesis and biological evaluation of quinazolin-4(3H)-one derivatives bearing dithiocarbamate side chain at C2-position as potential antitumor agents. Eur J Med Chem. 108:364-73




  23. 23.  Jie Tian, Chuan Tian, Yuehe Ding, Zhe Li, Qizhi Geng, Zhikai Xiahou, Jue Wang, Wenya Hou, Ji Liao, Meng-Qiu Dong, Xingzhi Xu* and Jing Li* 2015 Aurora B-dependent phosphorylation of Ataxin-10 promotes the interaction between Ataxin-10 and Plk1 in cytokinesis. Scientific Reports. 5 :8360




  24. 24.  Zhao H, Zhu M, Dou G, Zhao H, Zhu B, Li J, Liao J, Xu X. BCL10 regulates RNF8/RNF168-mediated ubiquitination in the DNA damage response. 2014 Cell Cycle. 13(11):1777-87.




  25. 25.  Jing Li1,*, Xiaoqian Liu1, Ji Liao, Jie Tian, Jue Wang, Xin Wang, Jiezhong Zhang and Xingzhi Xu*. 2013. MYPT1 sustains centromeric cohesion and the spindle-assembly checkpoint. Journal of Genetics and Genomics. 40(11):575-8.




  26. 26.  Jinping Liu, Linli Xu, Jianing Zhong, Ji Liao, Jing Li and Xingzhi Xu. 2012. Protein phosphatase PP4 is involved in NHEJ-mediated repair of DNA double-strand breaks. Cell Cycle 11(14), 2643-49 (Commentary on Cell Cycle 11(14), 2590)




  27. 27.  Jinping Liu, Shukun Luo, Hongchang Zhao, Ji Liao, Jing Li, Chunying Yang, Bo Xu, David F. Stern, Xingzhi Xu* and Keqiong Ye*. 2012. Structural mechanism of the phosphorylation-dependent dimerization of MDC1 forkhead-associated domain. Nucleic Acids Research 40(9):3898-3912 (featured article)




  28. 28.  Li J1,*., Wang, J1., Hou, W1., Jing, Z., Tian, C., Han, Y., Liao, J., Dong, M-Q., Xu, X*. 2011. Phosphorylation of Ataxin-10 by polo-like kinase 1 is required for cytokinesis. Cell Cycle 10(17):2946 – 2958 (cover article)




  29. 29.  Zhong J, Liao J, Liu X, Wang P, Liu J, Hou W, Zhu B, Yao L, Wang J, Li J, Stark JM, Xie Y, Xu X. 2011 Protein phosphatase PP6 is required for homology-directed repair of DNA double-strand breaks. Cell Cycle 10(9):1411-1419




  30. 30.  Lv, S., Bu, W., Jiao, H., Liu, B., Zhu, L., Zhao, H., Li, J., Liao, J., Xu, X. 2010. LSD1 is required for chromosome segregation during mitosis. Eur. J. Cell Biol. 89(7):557-63




  31. 31.  Li, J*., Wang, J., Jiao, H., Liao, J., and Xu, X*. 2010. Cytokinesis and cancer: Polo loves ROCK’n’Rho(A). Journal of genetics and genomics 37 (3):159-172



  32. 32.  Wang, H., Zhao, A., Chen, L., Zhong, X., Liao, J., Gao, M., Cai, M., Lee, D.H., Li, J., Chowdhury, D., Yang, Y.G., Pfeifer, G.P., Yen, Y., and Xu, X. 2009. Human RIF1 encodes an anti-apoptotic factor required for DNA repair. Carcinogenesis  30(8):1314-1319


  33. 33.  Zhang, X., Liu, D., Lv, S., Wang, H., Zhong, X., Liu, B., Wang, B., Liao, J., Li, J., Pfeifer G. P., and Xu, X. 2009. CDK5RAP2 is required for spindle checkpoint function. Cell Cycle 8(8): 1206-1216.



  34. 34.  Wang, B., Zhao, A., Sun, L., Zhong, X.. Zhong, J., Wang, H., Cai, M., Li, J., Xu, Y., Liao, J., Sang, J., Chowdhury, D., Pfeifer, G.P., Yen, Y., and Xu, X. 2008. Protein phosphatase PP4 is overexpressed in human breast and lung tumors. Cell Research 18:974-977




  35. 35.  Li, J., Agarwal, S., and Roeder, G.S. 2007 SSP2 and OSW1, Two sporulation-specific genes involved in spore morphogenesis in Saccharomyces cerevisiae. Genetics. 175(1):143-154




  36. 36. Li, J., Hooker, G.W., and Roeder, G.S. 2006. Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation. Genetics, 173(4): 1969-1981




  37. 37.  Huafei Lu, Xiaotian Ming, Lijia Qu, Meihua Liu, Jing Li, Hongya Gu, Zhangliang Chen. 2000. Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their expression in transgenic rice. Chinese Science Bulletin, 45(3):242-246


会议/口头报告

序号

会议名称

时间

地点

报告名称

1

EMBO conference “Replication/repair and segregation of chromosomes”

EMBO会议:DNA损伤,修复与染色体分离

2010.

6.12-17

Freiburg

Munzingen,Germany

A role for Plk1 phosphorylation of Ataxin-10 in cytokinesis


2

First Sino-German symposium on DNA repair and human disease 第一届中德DNA损伤与人类疾病研讨会

2010.

10.8-12


北京

Phosphorylation of Ataxin-10 by Plk1 is required for cytokinesis


3

北京细胞生物学会首届青年学者论坛

2013.

9.29

北京

Aurora B promotes the interaction between Ataxin-10 and Plk1 in cytokinesis

4

中国细胞生物学会

首届染色质会议

2017.

4.14-16

深圳

Plk1-dependent phosphorylation of MTHFR regulates S phase progression via H4K20me3

5

The 2nd International Symposium of Epigenetic Mechanism and Human Health第二届国际表观遗传学机制与人类健康大会

2017.

10.13-15

武汉

Plk1-dependent phosphorylation of MTHFR regulates S phase progression via histone methylation


6

The 11th International Symposium of Glycosyltransferase (GlycoT 2018) 国际糖基转移酶会议

2018.

6.19-23

青岛

Checkpoint kinase 1-induced phosphorylation of OGT regulates the intermediate filament network during cytokinesis

7

2018年全国糖生物学会

2018.

9.21-23

上海

O-GlcNAcylation antagonizes phosphorylation of Cdh1 (CDC20 homologue 1)

8

2019 ACS Orlando (257th)

257届美国化学学会2019春季年会 Chemical Biology of Glycoproteins 分会场

2019.3.31

Orlando, USA

O-GlcNAc: a sweetheart of the cell cycle

9

University of California-San Francisco 加州大学旧金山分校(UCSF)

2019.4.4

San Francisco, USA

O-GlcNAc: a sweetheart of the cell cycle

10

isDDRHD-2019

第十届国际DNA损伤与人类疾病研讨会
2019.11.8-10 深圳 UBE3D and KAP1: one mechanism, two diseases
11 第十一届全国化学生物学会议 2019.11.18-21 广州 Fantastic OGT and what does it do
12 北大医学青年科技沙龙 2020.6.23 腾讯会议 葡糖酰胺(O-GlcNAc)修饰--翻译后修饰的睡美人
13 2020年全国糖生物学会议 2020.9.19-21 无锡 Centrosomes: Til O-GlcNAc Do Us Apart
14 北大药学院天然药物及仿生药物国家重点实验室 2020.10.15 北京 O-GlcNAc: a sweetheart of the cell cycle
15 南开大学生命科学学院 2020.11.20 天津 O-GlcNAc: a sweetheart of the cell cycle
16 2021全国糖化学会议 2021.5.7-9 青岛 For better or for worse: Regulation of OGT under stress
17 云南大学生命科学学院 2021.7.5 昆明 O-GlcNAc: a sweetheart of the cell cycle